By accessing or using the Web Site, you agree that you have read, understand and agree to be bound by these Terms of Use, as amended from time to time, as well as the Company Privacy Policy, which is hereby incorporated into these Terms of Use. Please read these Terms of Use carefully before accessing or using any part of the Web Site. All users of the Web Site are subject to the following website terms and conditions of use (these “Terms of Use”). If you are accessing the Web Site from outside the United States, Canada, or Puerto Rico, please see the appropriate international website, available at for applicable terms and conditions. These terms and conditions of use are applicable to the United States, Canada and Puerto Rico websites (“collectively the Web Site”) operated by VWR (the “Company”). ![]() ![]() HALO® Chromatography Columns and Consumables+. Sartorius laboratory instruments, consumables and services PerkinElmer - Innovating for a Healthier World Lab Thermometers & Temperature Measurement EquipmentĪgilent Chemistries and Supplies Portfolio Quality Products from Sheldon Manufacturing Life Science Research Solutions, Products, and Resources VWR will support you from the latest life science products to the guaranteed purity of organic building blocks. Ready to make the switch to Stain-Free enabled precast gels? Use the Western Blot Selector Tool to easily find gels compatible for your next experiment.A strong, vibrant research and development group is the lifeblood of all industries. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations. Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS.
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